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Journal: Virology Journal
Article Title: Identification of linear B cell epitopes on the E146L protein of African swine fever virus with monoclonal antibodies
doi: 10.1186/s12985-024-02570-0
Figure Lengend Snippet: Preliminary epitope mapping of mAbs. ( A ) The outer-membrane domain of the E146L protein was truncated into seven short peptides 30 amino acids in length with 15 amino acids overlapping. IMD: internal membrane domain, TMD: transmembrane domain. ( B ) The peptides were expressed as MBP fusion proteins in Escherichia coli and verified by SDS‒PAGE. The peptide fusion proteins were used to map the epitopes of the mAbs by ELISA ( C , E and G ) and western blotting ( D , F and H ). The results revealed that the mAb 12H12 recognized peptides P1 and P2, and the mAbs 15G1 and 15H10 identified peptides P7 and P1, respectively
Article Snippet: Six transmembrane proteins of ASFV (I329L, E146L, C257L, EP153R, I177L, and F165R) were selected through the
Techniques: Membrane, Enzyme-linked Immunosorbent Assay, Western Blot